Plant Biotechnology
Plant biotechnology is a major branch of biotechnology. It may be defined as the manipulation of genetic engineering and plant tissue culture techniques for genetic modifications under consideration of specific purposes. The specific purposes may be development of transgenic crop plants, disease resistant crop varieties, somaclonal variants, bio-fortified crops , abiotic stress tolerance, altered growth/yield, herbicide tolerance, insect resistance, modified product quality and so on. The development of virus free plant from small explants through plant cell and tissue culture is a major dimension in plant biotechnology. Plant cell and tissue culture helps in transgenic plant development .Because in plant biotechnology transgenics followed by plant tissue culture techniques. Plant biotechnology usually employed in the field of agriculture and molecular pharming. In modern breeding program plant biotechnology also plays significant roles. Because modern biotechnology respect the breeding program at molecular levels.
Plant products of biotechnology approved for food use have been qualitative and quantitative modified to contain traits such as:
- Insect resistance
- Disease resistance
- Herbicide tolerance
- Modified nutritional profile
- bio-fortified crop development
Table of contents :
| Introduction :Plant biotechnology | Secondary metabolites production from PTC |
| Terminology | Germplasm conservation |
| Introductory history | PTC for crop improvement |
| Laboratory requirements and general techniques | Transgenic plant development |
| Tissue culture media composition & preparation | PTC related research institution and commercial companies |
| Cell culture | Genetic engineering |
| Cellular totipotency | Restriction enzymes |
| Somatic embryogenesis | Vectors for gene transfer |
| Haploid production | Gene cloning |
| Triploid production | Transgenic plants and their developments |
| Variant selection | QTL mapping |
| In vitro pollination and fertilization | MAS and its application |
| Zygotic Embryo culture & embryo rescue | |
| Protoplast isolation, fusion ,and culture | |
| Somatic hybridization & cybridization | |
| Clonal or micro-propagation | |
| Artificial seeds(synseeds/synthetic seeds ) | |
| Somatic embryogenesis principles , concepts & appllications | |
| Anther culture / pollen culture | |
| Ovary culture , ovule culture , emdosperm culture |