Molecular Biology
|
S. No. |
Topic |
|
1. |
Organization of bacterial genome |
|
2. |
Structure of eukaryotic chromosomes Heterochromatin and Euchromatin |
|
3. |
Role of nuclear matrix in chromosome and function |
|
4. |
DNA re-association kinetics (cot curve analysis) |
|
5. |
Repetitive and unique sequences: Satellite DNA |
|
6. |
DNA melting and buoyant density: Nucleosome phasing |
|
7. |
DNA’s Hypersensitive regions. DNA methylation & imprinting |
|
8. |
Structure of DNA; A-, B-, Z- and triplex DNA |
|
9. |
DNA metabolism |
|
10 |
Replication, mutation, elongation and termination in prokaryotes and eukaryotes |
|
11 |
Enzymes and accessory proteins, Fidelity |
|
12 |
Replication of single stranded circular DNA |
|
13 |
Gene stability |
|
14 |
DNA repair enzymes, Photoreactivation |
|
15 |
Nucleotide excision repair, Mismatch correction |
|
16 |
SOS repair |
|
17 |
Recombination Homologous and Non homologous, Site specific recombination, Chi sequences in Prokaryoties |
|
18 |
ELP HRT and Cre/Lox recombination |
|
19 |
Gene targeting, Gene disruption |
|
20 |
Prokaryotic and eukaryotic Transcription, Transcription unit |
|
21 |
Promoters Constitutive and inducible: Operators, Regulatory elements |
|
22 |
Limitation, Attenuation, Termination-Rho-dependent and independent, Anti-termination |
|
23 |
Transcriptional regulation-Positive and Negative |
|
24 |
Operon concept, lac, trp, ara, his and gal operons |
|
25 |
Transcriptional concept lambda phage |
|
26 |
Transcript processing, Processing of tRNA and rRNA Eucaryotic transcription and regulation |
|
27 |
RNA polymerase structure and assembly, RNA polymerase I, II, II |
|
28 |
Eukaryotic promoters and enhancers |
|
29 |
General Transcription factors, TATA binding proteins (TBP) and TPB associated factors (TAF) |
|
30 |
Activators and repressors |
|
31 |
Transcriptional and post transcriptional gene silencing |
|
32 |
Processing of hnRNA , tRNA, rRNA |
|
33 |
5’ Cap formation, 3’ end processing and polyadenylation |
|
34 |
Splicing |
|
35 |
RNA editing |
|
36 |
Nuclear export of mRNA stability |
|
37 |
Catalytic RNA |
|
38 |
Translation machinery, Ribosomes, Composition and Assembly, isoaccepting tRNA |
|
39 |
Universal genetic code, Degeneracy of codons, Termination codons, Wobble hypothesis, Genetic code in mitochondria |
|
40 |
Translation, Mechanism of mutation, elongation and termination |
|
41 |
Co and post translation modification |
|
42 |
Transport of protein and molecular chaperones |
|
43 |
Protein stability, Protein turnover and degradation |
|
44 |
Nonsense, missense and point mutation, Intragenic and Intergenic suppression: Frame shift mutation |
|
45 |
Physical, Chemical and Biological mutagens |
|
46 |
Transposition, Transposable genetic elements in prokaryotes and eukaryotes |
|
47 |
Mechanisms of transposition |
|
48 |
Role of transposon in mutation |
|
49 |
Viral and cellular oncogenes |
|
50 |
Tumor suppressor genes from humans, Structure, function and mechanism of action of pRB and p53 tumor suppressor proteins. |
|
51 |
Activation of oncogenes and dominant negative effect, Suppression of tumor suppressor genes |
|
52 |
Oncogenes as transcriptional activators |