Molecular Biology

S. No.

Topic

1.

Organization of bacterial genome

2.

Structure of eukaryotic chromosomes Heterochromatin and Euchromatin

3.

Role of nuclear matrix in chromosome and function

4.

DNA re-association kinetics (cot curve analysis)

5.

Repetitive and unique sequences: Satellite DNA

6.

DNA melting and buoyant density: Nucleosome phasing

7.

DNA’s Hypersensitive regions. DNA methylation & imprinting

8.

Structure of DNA; A-, B-, Z- and triplex DNA

9.

DNA metabolism

10

Replication, mutation, elongation and termination in prokaryotes and eukaryotes

11

Enzymes and accessory proteins, Fidelity

12

Replication of single stranded circular DNA

13

Gene stability

14

DNA repair enzymes, Photoreactivation

15

Nucleotide excision repair, Mismatch correction

16

SOS repair

17

Recombination Homologous and Non homologous, Site specific recombination,  

Chi sequences in Prokaryoties

18

ELP HRT and Cre/Lox recombination

19

Gene targeting, Gene disruption

20

Prokaryotic  and eukaryotic Transcription, Transcription unit

21

Promoters Constitutive and inducible: Operators, Regulatory elements

22

Limitation, Attenuation, Termination-Rho-dependent and independent, Anti-termination

23

Transcriptional regulation-Positive and Negative

24

Operon concept, lac, trp, ara, his and gal operons

25

Transcriptional concept lambda phage

26

Transcript processing, Processing of tRNA and rRNA Eucaryotic transcription and regulation

27

RNA polymerase structure and assembly, RNA polymerase I, II, II

28

Eukaryotic promoters and enhancers

29

General Transcription factors, TATA binding proteins (TBP) and TPB associated factors (TAF)

30

Activators and repressors

31

Transcriptional and post transcriptional gene silencing

32

Processing of hnRNA , tRNA, rRNA

33

5 Cap formation, 3 end processing and polyadenylation

34

Splicing

35

RNA editing

36

Nuclear export of mRNA stability

37

Catalytic RNA

38

Translation machinery, Ribosomes, Composition and Assembly, isoaccepting tRNA  

39

Universal genetic code, Degeneracy of codons, Termination codons, Wobble hypothesis, Genetic code in mitochondria

40

Translation, Mechanism of mutation, elongation and termination

41

Co and post translation modification

42

Transport of protein and molecular chaperones

43

Protein stability, Protein turnover and degradation

44

Nonsense, missense and point mutation, Intragenic and Intergenic suppression: Frame shift mutation

45

Physical, Chemical and Biological mutagens

46

Transposition, Transposable genetic elements in prokaryotes and eukaryotes

47

Mechanisms of transposition

48

Role of transposon in mutation

49

Viral and cellular oncogenes

50

Tumor suppressor genes from humans, Structure, function and mechanism of action of pRB and p53 tumor suppressor proteins.

51

Activation of oncogenes and dominant negative effect, Suppression of tumor suppressor genes

52

Oncogenes as transcriptional activators