Recombinant DNA technology

  • Recombinant DNA technology refers to the use of sets of molecular techniques and restriction enzymes for recombining genes or regulatory sequences from different  bio -sources and to make r-DNA in-vitro and transferring this by several means into cells where it may be expressed or cloned for specific purposes.

  • RDT is a major technology used in genetic engineering.

  • RDT is the soul of genetic engineering.

  • Genetic engineering is the pillar of modern biotechnology.

  • In terms of genetic modification, RDT is the mimicking of the natural genetic recombination.

Recombinant DNA technology is also based on central dogma of Molecular Biology because of r-DNA transcribed into mRNA and these mRNA get translated into recombinant protein( r-protein). There are various objectives of RDT but development of transgenic micro-organisms, plants or animals for particular purpose. The expression of recombinant DNA (gene) into novel protein is based on the central dogma of Molecular Biology.

# Topic related to Recombinant DNA Technology:

RDT: Tools and Techniques
Restriction endonucleases: Types and uses
DNA ligases
Vectors: A brief introduction
Plasmids
Cosmids
Phagemids
BACs
PACs,
YACs
Role of microbes in RDT
Transposon vectors
Expression vectors
Shuttle vectors
Binary plant vectors
 Co-integrating vectors
Competent cells
Gene isolation and cloning
Genetic transformation of E. coli
Gel electrophoresis
Preparation of probes
Southern blotting
Northern blotting
Western blotting

Appendix 1.0      Appendix 1.1    Appendix 1.2       Appendix 1.3       Appendix 1.4       Appendix 1.5      Appendix 1.6    Appendix 1.7       Appendix 1.8