Recombinant DNA technology
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Recombinant DNA technology refers to the use of sets of molecular techniques and restriction enzymes for recombining genes or regulatory sequences from different bio -sources and to make r-DNA in-vitro and transferring this by several means into cells where it may be expressed or cloned for specific purposes.
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RDT is a major technology used in genetic engineering.
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RDT is the soul of genetic engineering.
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Genetic engineering is the pillar of modern biotechnology.
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In terms of genetic modification, RDT is the mimicking of the natural genetic recombination.
Recombinant DNA technology is also based on central dogma of Molecular Biology because of r-DNA transcribed into mRNA and these mRNA get translated into recombinant protein( r-protein). There are various objectives of RDT but development of transgenic micro-organisms, plants or animals for particular purpose. The expression of recombinant DNA (gene) into novel protein is based on the central dogma of Molecular Biology.
# Topic related to Recombinant DNA Technology:
| RDT: Tools and Techniques |
| Restriction endonucleases: Types and uses |
| DNA ligases |
| Vectors: A brief introduction |
| Plasmids |
| Cosmids |
| Phagemids |
| BACs |
| PACs, |
| YACs |
| Role of microbes in RDT |
| Transposon vectors |
| Expression vectors |
| Shuttle vectors |
| Binary plant vectors |
| Co-integrating vectors |
| Competent cells |
| Gene isolation and cloning |
| Genetic transformation of E. coli |
| Gel electrophoresis |
| Preparation of probes |
| Southern blotting |
| Northern blotting |
| Western blotting |
Appendix 1.0 Appendix 1.1 Appendix 1.2 Appendix 1.3 Appendix 1.4 Appendix 1.5 Appendix 1.6 Appendix 1.7 Appendix 1.8