Recombinant DNA technology
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Recombinant DNA technology refers to the use of sets of molecular techniques and restriction enzymes for recombining genes or regulatory sequences from different bio -sources and to make r-DNA in-vitro and transferring this by several means into cells where it may be expressed or cloned for specific purposes.
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RDT is a major technology used in genetic engineering.
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RDT is the soul of genetic engineering.
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Genetic engineering is the pillar of modern biotechnology.
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In terms of genetic modification, RDT is the mimicking of the natural genetic recombination.

Recombinant DNA technology is also based on central dogma of Molecular Biology because of r-DNA transcribed into mRNA and these mRNA get translated into recombinant protein( r-protein). There are various objectives of RDT but development of transgenic micro-organisms, plants or animals for particular purpose. The expression of recombinant DNA (gene) into novel protein is based on the central dogma of Molecular Biology.
# Topic related to Recombinant DNA Technology:
RDT: Tools and Techniques |
Restriction endonucleases: Types and uses |
DNA ligases |
Vectors: A brief introduction |
Plasmids |
Cosmids |
Phagemids |
BACs |
PACs, |
YACs |
Role of microbes in RDT |
Transposon vectors |
Expression vectors |
Shuttle vectors |
Binary plant vectors |
Co-integrating vectors |
Competent cells |
Gene isolation and cloning |
Genetic transformation of E. coli |
Gel electrophoresis |
Preparation of probes |
Southern blotting |
Northern blotting |
Western blotting |
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