Recombinant DNA technology

  • Recombinant DNA technology refers to the use of sets of molecular techniques and restriction enzymes for recombining genes or regulatory sequences from different  bio -sources and to make r-DNA in-vitro and transferring this by several means into cells where it may be expressed or cloned for specific purposes.

  • RDT is a major technology used in genetic engineering.

  • RDT is the soul of genetic engineering.

  • Genetic engineering is the pillar of modern biotechnology.

  • In terms of genetic modification, RDT is the mimicking of the natural genetic recombination.

Recombinant DNA technology is also based on central dogma of Molecular Biology because of r-DNA transcribed into mRNA and these mRNA get translated into recombinant protein( r-protein). There are various objectives of RDT but development of transgenic micro-organisms, plants or animals for particular purpose. The expression of recombinant DNA (gene) into novel protein is based on the central dogma of Molecular Biology.

# Topic related to Recombinant DNA Technology:

RDT: Tools and Techniques
Restriction endonucleases: Types and uses
DNA ligases
Vectors: A brief introduction
Plasmids
Cosmids
Phagemids
BACs
PACs,
YACs
Role of microbes in RDT
Transposon vectors
Expression vectors
Shuttle vectors
Binary plant vectors
 Co-integrating vectors
Competent cells
Gene isolation and cloning
Genetic transformation of E. coli
Gel electrophoresis
Preparation of probes
Southern blotting
Northern blotting
Western blotting

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